Barrie Ingham character Basil of Baker Street (voice)
Val Bettin character Dr. David Q. Dawson (voice)
Vincent Price character Professor Ratigan (voice)
Susanne Pollatschek character Olivia Flaversham (voice)
Candy Candido character Fidget (voice)
Diana Chesney character Mrs. Judson (voice)
Eve Brenner character The Mouse Queen (voice)
Alan Young character Hiram Flaversham (voice)
Basil Rathbone character Sherlock Holmes (voice)
Ellen Fitzhugh character Bar Maid (voice)
Walker Edmiston character Citizen (voice)
Wayne Allwine character Thug Guard (voice)
Tony Anselmo character Thug Guard (voice)
Frank Welker character Toby the Dog/Felicia the Cat (voice) (uncredited)
Laurie Main character Dr. Watson (archive sound)
Shani Wallis character Lady Mouse (voice)
Melissa Manchester character Miss Kitty Mouse (voice)
Charles Fleischer character (voice) (uncredited)
