Freddie Prinze Jr. character Fred Jones
Sarah Michelle Gellar character Daphne Blake
Matthew Lillard character Norville "Shaggy" Rogers
Linda Cardellini character Velma Dinkley
Seth Green character Patrick Wisely
Peter Boyle character Old Man Wickles
Tim Blake Nelson character Jacobo
Alicia Silverstone character Heather
Kevin Durand character Black Knight Ghost
Neil Fanning character Scooby-Doo (voice)
Bill Meilen character Chauffeur
Chris Gauthier character Daphne's Tattooed Fan #1
Peter New character Daphne's Tattooed Fan #2
Calum Worthy character Kid on Bike #2
Colin Foo character Investor
Pat O'Brien character Pat O'Brien
Zahf Paroo character Ned
C. Ernst Harth character Miner 49er
Stephen E. Miller character C.L. Magnus
Ted Kozma character Museum Guard
Lisa Ann Beley character Mullet Nerdette #2
Scott McNeil character Evil Masked Figure (voice)
Ruben Studdard character Ruben Studdard
Bob Papenbrook character Black Knight Ghost (voice)
Dee Bradley Baker character 10,000 Volt Ghost / Zombie / Red Eye Skeleton (voice)
Michael Sorich character Tar Monster / Cotton Candy Glob (voice) (as Michael J. Sorich)
Terrence Stone character 10,000 Volt Ghost (voice)
Wally Wingert character Green-Eyed Skeleton (voice)
J.P. Manoux character Brainiac Scooby (voice)
Brandon Jay McLaren character Skater Dude #2
Michael Buffer character (voice)
