Will Arnett character Batman / Bruce Wayne (voice)
Rosario Dawson character Batgirl / Barbara Gordon (voice)
Mariah Carey character Mayor McCaskill (voice)
Ralph Fiennes character Alfred Pennyworth (voice)
Michael Cera character Robin / Dick Grayson (voice)
Zach Galifianakis character The Joker (voice)
Jenny Slate character Harley Quinn (voice)
Billy Dee Williams character Two-Face / Harvey Dent (voice)
Héctor Elizondo character Jim Gordon (voice)
Conan O'Brien character The Riddler / Edward Nygma (voice)
Jason Mantzoukas character Scarecrow / Jonathan Crane (voice)
Doug Benson character Bane (voice)
Zoë Kravitz character Catwoman / Selina Kyle (voice)
Kate Micucci character Clayface / Basil Karlo (voice)
Riki Lindhome character Poison Ivy / Pamela Isley (voice)
Channing Tatum character Superman / Clark Kent (voice)
Jonah Hill character Green Lantern / Hal Jordan (voice)
Adam Devine character The Flash / Barry Allen (voice)
Eddie Izzard character Voldemort (voice)
Seth Green character King Kong (voice)
Jemaine Clement character Sauron (voice)
Ellie Kemper character Phyllis (voice)
David Burrows character Anchorman Phil (voice)
Ralph Garman character Reporter #2 (voice)
Chris Hardwick character Reporter (voice)
Chris McKay character Pilot Bill (voice)
Laura Kightlinger character Reporter Pippa (voice)
Susan Bennett character Batcomputer (voice)
Todd Hansen character Captain Dale (voice)
Mark Jonathan Davis character Richard Cheese (voice)
Lauren White character Chief O'Hara / Medusa / Additional Voices (voice)
Brent Musburger character Reporter #1 (voice)
Abel Arnett character Additional Voices (voice)
Archie Arnett character Additional Voices (voice)
Marco Campagno character Additional Voices (voice)
Garret Elkins character Additional Voices (voice)
Ryan Halprin character Additional Voices (voice)
Alison Ingram character Additional Voices (voice)
Holly Isaacson character Additional Voices (voice)
Kelly Lafferty character Additional Voices (voice)
Doug Nicholas character Additional Voices (voice)
Samantha Nisenboim character Additional Voices (voice)
Chris Paluszek character Additional Voices (voice)
Wendell Stephens character Additional Voices (voice)
Vanara Taing character Additional Voices (voice)
Matt Town character Additional Voices (voice)
Bram Tulloch character Additional Voices (voice)
John Venzon character Penguin
Matt Villa character Additional Voices (voice)
Charlie Bean character Additional Voices (voice)