Shameik Moore character Miles Morales / Spider-Man (voice)
Hailee Steinfeld character Gwen Stacy / Spider-Woman (voice)
Jason Schwartzman character Jonathan Ohnn / Spot (voice)
Oscar Isaac character Miguel O'Hara / Spider-Man 2099 (voice)
Brian Tyree Henry character Jeff Morales (voice)
Luna Lauren Velez character Rio Morales (voice)
Jake Johnson character Peter B. Parker / Spider-Man (voice)
Issa Rae character Jessica Drew / Spider-Woman (voice)
Karan Soni character Pavitr Prabhakar / Spider-Man India (voice)
Shea Whigham character George Stacy (voice)
Greta Lee character LYLA (voice)
Amandla Stenberg character Margo Kess: Spider-Byte (voice)
Jharrel Jerome character Miles G. Morales (voice)
Andy Samberg character Ben Reilly / Scarlet Spider (voice)
Jack Quaid character Peter Parker / The Lizard (voice)
Rachel Dratch character Ms. Weber (voice)
Ziggy Marley character Lenny (voice)
Jorma Taccone character Adriano Tumino / '67 (voice)
Daniel Kaluuya character Hobie Brown / Spider-Punk (voice)
Mahershala Ali character Uncle Aaron (voice)
J.K. Simmons character J. Jonah Jameson (voice)
Donald Glover character Aaron Davis
Elizabeth Perkins character May / Quippy Spider-Person (voice)
Kathryn Hahn character Doc Ock (voice)
Ayo Edebiri character Glory (voice)
Nicole Delaney character MJ (voice)
Antonina Lentini character Betty (voice)
Atsuko Okatsuka character Yuri (voice)
Peter Sohn character Ganke (voice)
Melissa Sturm character Mary Jane (voice)
Lorraine Velez character Maria (voice)
Nic Novicki character Lego Spider-Man (voice)
Taran Killam character Web-Slinger (voice)
Metro Boomin character Metro Spider-Man (voice)
Josh Keaton character Spectacular Spider-Man (voice)
Sofia Barclay character Malala Windsor: Spider-UK (voice)
Danielle Perez character Charlotte Webber: Sun-Spider (voice)
Yuri Lowenthal character Insomniac Spider-Man (voice)
Rita Rani Ahuja character Additional Voices (voice)
Ismail Bashey character Additional Voices (voice)
Oscar Camacho character Additional Voices (voice)
Freddy Ferrari character Additional Voices (voice)
Kerry Gutierrez character Additional Voices (voice)
Kamal Khan character Additional Voices (voice)
Angelo Sekou Kouyate character Additional Voices (voice)
Andrew Leviton character Additional Voices (voice)
David Michie character Additional Voices (voice)
Sumit Naig character Additional Voices (voice)
Juan Pacheco character Additional Voices (voice)
Chrystee Pharris character Additional Voices (voice)
Ben Pronsky character Additional Voices (voice)
Al Rodrigo character Additional Voices (voice)
Stan Sellers character Additional Voices (voice)
Warren Sroka character Additional Voices (voice)
Jasper Johannes Andrews character Additional Voices (voice)
Gredel Berrios Calladine character Additional Voices (voice)
Natalia Castellanos character Additional Voices (voice)
Russell Tyre Francis character Additional Voices (voice)
Deepti Gupta character Additional Voices (voice)
Sohm Kapila character Additional Voices (voice)
Pradnya Kuwadekar character Additional Voices (voice)
Ashley London character Additional Voices (voice)
Christopher Miller character Additional Voices (voice)
Andrea Navedo character Additional Voices (voice)
Lakshmi Patel character Additional Voices (voice)
Jacqueline Pinol character Additional Voices (voice)
Eliyas Qureshi character Additional Voices (voice)
Lashana Rodriguez character Additional Voices (voice)
Jaswant Dev Shrestha character Additional Voices (voice)
Libby Thomas Dickey character Additional Voices (voice)
Jason Linere-White character Additional Voices (voice)
Sitara Attaie character Additional Voices (voice)
Mayuri Bhandari character Additional Voices (voice)
June Christopher character Additional Voices (voice)
Michelle Jubilee Gonzalez character Additional Voices (voice)
Marabina Jaimes character Additional Voices (voice)
Rez Kempton character Additional Voices (voice)
Lex Lang character Additional Voices (voice)
Phil Lord character Additional Voices (voice)
Richard Miro character Additional Voices (voice)
Doug Nicholas character Additional Voices (voice)
Shakira Ja'nai Paye character Additional Voices (voice)
James Pirri character Additional Voices (voice)
Marley Ralph character Additional Voices (voice)
Michelle Ruff character Additional Voices (voice)
Dennis Singletary character Additional Voices (voice)
Amanda Troop character Additional Voices (voice)
Ruth Zalduondo character Additional Voices (voice)
Kimberly Bailey character Additional Voices (voice)
Sanjay Chandani character Additional Voices (voice)
Melanie Duke character Additional Voices (voice)
Jorge R. Gutierrez character Additional Voices (voice)
Miguel Jiron character Additional Voices (voice)
Deepti Kingra-Mickelsen character Additional Voices (voice)
Luisa Leschin character Additional Voices (voice)
Caitlin McKenna character Additional Voices (voice)
Andrew Morgado character Additional Voices (voice)
Arthur Ortiz character Additional Voices (voice)
Eliana A. Perez character Additional Voices (voice)
Juan Pope character Additional Voices (voice)
Mike Rianda character Additional Voices (voice)
Erika Scopelli character Additional Voices (voice)
Narender Sood character Additional Voices (voice)
Cedric L. Williams character Additional Voices (voice)
Kimiko Glenn character Peni Parker (voice) (uncredited)
Peggy Lu character Mrs. Chen (archive footage) (uncredited)
Andrew Garfield character Peter Parker / Spider-Man (archive footage) (uncredited)
Denis Leary character Captain Stacy (archive footage) (uncredited)
Tobey Maguire character Peter Parker / Spider-Man (archive footage) (uncredited)
Cliff Robertson character Ben Parker (archive footage) (uncredited)
Alfred Molina character Doctor Octopus (voice) (archive footage) (uncredited)
Post Malone character Brooklyn Bystander (voice) (archive footage) (uncredited)
