Kevin James character Otis the Cow (voice)
Courteney Cox character Daisy the Cow (voice)
Sam Elliott character Ben the Cow (voice)
Danny Glover character Miles the Mule (voice)
Wanda Sykes character Bessy the Cow (voice)
Andie MacDowell character Etta the Hen (voice)
David Koechner character Dag the Coyote (voice)
Jeffrey Garcia character Pip the Mouse (voice)
Cam Clarke character Freddy the Ferret (voice)
Rob Paulsen character Peck the Rooster / Gopher (voice)
Tino Insana character Pig the Pig (voice)
Dom Irrera character Duke the Dog (voice)
S. Scott Bullock character Eddy the Cow (voice)
John DiMaggio character Bud the Cow / Officer O'Hanlon (voice)
Maurice LaMarche character Igg the Cow (voice)
Madeline Lovejoy character Maddy the Chick (voice)
Earthquake character Root the Rooster (voice)
Steve Oedekerk character Snotty Boy / Mr. Beady / Snotty Boy's Father (voice)
Maria Bamford character Mrs. Beady (voice)
Fred Tatasciore character Farmer (voice)
Peter Stenstrom character Skunk (voice)
Jill Talley character Snotty Boy's Mother (voice)
Laraine Newman character Snotty Boy's Friend (voice)
Katie Leigh character Snotty Boy's Friend / Additional Barnyard Voices (voice)
North Mississippi Allstars character Barn Boys (voice)
Anna Pistor character Chick (voice)
Thomas Pistor character Chick (voice)
Eliana Bendetson character Chick (voice)
Paul Butcher character Chick (voice)
Khamani Griffin character Chick (voice)
Arlo Levin character Chick (voice)
Liliana Mumy character Chick (voice)
Cydney Neal character Chick (voice)
Cate Ozawa character Chick (voice)
Isaiah Tefilo character Chick (voice)
Georgia Van Newkirk character Chick (voice)
Joni Allen character Singer (voice)
Leigh French character Additional Barnyard Voices (voice)
William Calvert character Additional Barnyard Voices (voice)
Julianne Buescher character Additional Barnyard Voices (voice)
Nicholas Guest character Additional Barnyard Voices (voice)
Audrey Wasilewski character Additional Barnyard Voices (voice)
Phil Proctor character Additional Barnyard Voices (voice)
Archie Hahn character Additional Barnyard Voices (voice)
Chad Einbinder character Additional Barnyard Voices (voice)
Keith Anthony character Additional Barnyard Voices (voice)
Claudette Wells character Additional Barnyard Voices (voice)
Lynne Marie Stewart character Additional Barnyard Voices (voice)
Christie Mellor character Additional Barnyard Voices (voice)
Justin Shenkarow character Additional Barnyard Voices (voice)
Jacqueline Pinol character Additional Barnyard Voices (voice)
Nika Futterman character Additional Barnyard Voices (voice)
Eddie Frierson character Additional Barnyard Voices (voice)