Jason Lee character David Seville
David Cross character Ian
Jenny Slate character Zoe
Justin Long character Alvin (voice)
Matthew Gray Gubler character Simon (voice)
Jesse McCartney character Theodore (voice)
Amy Poehler character Eleanor (voice)
Anna Faris character Jeanette (voice)
Christina Applegate character Brittany (voice)
Alan Tudyk character Simone (voice)
Michael P. Northey character Hawaiian Shirt Guy
Sophia Aguiar character Club Woman #1
Lauren Gottlieb character Club Woman #2
Tera Perez character Club Woman #3
Andy Buckley character Captain Correlli
Chad Krowchuk character Waiter
Luisa D'Oliveira character Tessa
Tucker Albrizzi character Tucker / Kite Kid
Nelson Wong character Casino Guy
Michael Bagdasarian character Excursion Clerk
Amber-Rochelle DeMarco character Damsel #1
Marcela Caceres character Damsel #2
Natalie Sutcliffe character Damsel #3
Ian Harmon character Monster
Phyllis Smith character Flight Attendant
Ross Bagdasarian, Jr. character Alvin (singing voice) (uncredited)
Janice Karman character Theodore / The Chipettes (singing voice) (uncredited)
Steve Vining character Simon (singing voice) (uncredited)
Aaron Atkins character Casino Dealer (uncredited)
Eoin Bates character Music Executive (uncredited)
Seth Robert Dusky character Additional Voices (voice) (uncredited)
Wendell Kinney character Cruise Guest (uncredited)
Jeremy Palko character Cruise Guy (uncredited)
Rachael Wegener character Model (uncredited)