Megan Fox character April O'Neil
Will Arnett character Vernon Fenwick
William Fichtner character Eric Sacks
Alan Ritchson character Raphael
Noel Fisher character Michelangelo
Pete Ploszek character Leonardo
Johnny Knoxville character Leonardo (voice)
Jeremy Howard character Donatello
Danny Woodburn character Master Splinter
Tony Shalhoub character Splinter (voice)
Tohoru Masamune character Shredder
Whoopi Goldberg character Bernadette Thompson
Minae Noji character Karai
Abby Elliott character Taylor
Madison Mason character Councilman
Derek Mears character Dojo Ninja
Malina Weissman character Young April O'Neil
Taran Killam character McNaughton
K. Todd Freeman character Dr. Baxter Stockman
Paul Fitzgerald character Dr. O'Neil
Venida Evans character Subway Hostage
Mikal Vega character Sacks Bodyguard
Harley Pasternak character Himself
Braeson Herold character Reporter
Chance Kelly character Mr. Rivetti
Rick Chambers character News Anchor
Leyna Nguyen character News Anchor
Chris Wylde character Reporter