Bob Hoskins character Eddie Valiant
Christopher Lloyd character Judge Doom
Joanna Cassidy character Dolores
Charles Fleischer character Roger Rabbit/Benny The Cab/Greasy/Psycho (voice)
Kathleen Turner character Jessica Rabbit (voice) (uncredited)
Stubby Kaye character Marvin Acme
Alan Tilvern character R.K. Maroon
Richard LeParmentier character Lt. Santino
Lou Hirsch character Baby Herman (voice)
Betsy Brantley character Jessica's Performance Model
Mel Blanc character Bugs Bunny/Daffy Duck/Tweety Bird (voice)
Mae Questel character Betty Boop (voice)
Frank Sinatra character Singer
Richard Williams character Droopy (voice)
Wayne Allwine character Mickey Mouse (voice)
Frank Welker character Dumbo (voice)
Mickie McGowan character Mooing Cow/Flying Witch (voice)
Jack Angel character Additional Voices (voice)
Nancy Cartwright character Dipped Shoe (voice)
June Foray character Wheezy/Lena Hyena (voice)
Joel Silver character Raoul
Amy Irving character Jessica Rabbit (singing voice)
Paul Springer character Augie
Richard Ridings character Angelo
Edwin Craig character Arthritic Cowboy
Lindsay Holiday character Soldier
Mike Edmonds character Stretch
Morgan Deare character Editor / Gorilla (voice)
Danny Capri character Kid #1
Christopher Hollosy character Kid #2
Jean-Paul Sipla character Kid #3
Laura Frances character Blonde Starlet
Joel Cutrara character Forensic #1
Billy J. Mitchell character Forensic #2
Eric B. Sindon character Mailman
Ed Herlihy character Newscaster
James O'Connell character Conductor
Eugene Gutierrez character Teddy Valiant
April Winchell character Mrs. Herman (voice)
Tony Anselmo character Donald Duck (voice)
Mary T. Radford character Hippo (voice)
Joe Alaskey character Yosemite Sam / Foghorn Leghorn (voice)
David L. Lander character Smart Ass (voice)
Fred Newman character Stupid (voice)
June Foray character Wheezy / Lena Hyena (voice)
Russi Taylor character Birds / Minnie Mouse (voice)
Les Perkins character Mr. Toad (voice)
Richard Williams character Droopy Dog
Pat Buttram character Bullet #1
Jim Cummings character Bullet #2
Jim Gallant character Bullet #3
Tony Pope character Goofy / Big Bad Wolf (voice)
Peter Westy character Pinocchio (voice)
Cherry Davis character Woody Woodpecker
