Paul Reubens character Pee-wee Herman
Jordan Black character Yul / Party Coordinator (voice)
Doug Cox character Mr. Murgatroyd
Linda Porter character Mrs. Rose
Monica Horan character Ruby the Crossing Guard
Brian Palermo character Marvin
Katherine Vanderlinden character Emily
Tara Buck character Beverly
John H. Mayer character Pharmacist
Richard Riehle character Dan
Dave Power character Dudley
Josh Meyers character Sylvester
Corey Craig character Clovis
Paul Rust character Ernie
Robert R. Shafer character Construction worker
John Moody character Construction Worker
Joe Manganiello character Joe Manganiello
Jessica Pohly character Pepper
Stephanie Beatriz character Freckles
Alia Shawkat character Bella
Daniel Lue character Police Officer
Darrin Henson character Police Officer
Clayton Cannon character Police Officer
Patrick Egan character Gordon the Traveling Salesman
Gloria Vassy character Panicked Female Passenger
Erick Chavarria character Panicked Male Passenger
Frank Collison character Clay
Lynne Marie Stewart character Jimmy
Karen Maruyama character Tourist at Snake Farm
Hal Landon Jr. character Farmer Brown
Michelle Meredith character Linda Brown
Meredith O'Leary character Louise Brown
K.T. Wiegman character Donna Brown
Julie Ouellette character Diane Brown
Alexandra Ella character Janice Brown
Mary Karcz character Betty Brown
Lily Mae Harrington character Pam Brown
Mackenzie Marsh character Judy Brown
Lindsay Hollister character Peggy Brown
Cooper Huckabee character Minister
Darryl Stephens character Rene
Dionne Gipson character Brook
Anthony Alabi character Lane
Sonya Eddy character Lane
Diane Salinger character Penny King
Brad William Henke character Grizzly Bear Daniels
Leo Fitzpatrick character Abe
Christopher Heyerdahl character Ezekiel
Alan R. Rodriguez character New York City Policeman
Michael Pauley character Guy who bumps into Pee-wee (as Michael R. Pauley)
Joseph Mancuso character Pizzeria Employee
David Arquette character DJ
Janina Gavankar character Party Guest
Kelly Bush Novak character Party Guest
Taymour Ghazi character Party Guest
Nicole Sullivan character Shelly
Robert Broski character Abraham Lincoln
Pamela Mant character Queen
Thea Andrews character TV Reporter
John Paragon character TV Cameraman
Charles Robinson character Police Captain (as Charlie Robinson)
Michael Acosta character Extra (uncredited)
Vanessa Alameda character Jailbird (uncredited)
Maya Nalli character Waitress
Deon Aumaier character Man with Toupee (uncredited)
