Eddie Murphy character Dr. John Dolittle
Kristen Wilson character Lisa Dolittle
Kyla Pratt character Maya Dolittle
Raven-Symoné character Charisse Dolittle
Steve Zahn character Archie (voice)
Lisa Kudrow character Ava (voice)
James Avery character Eldon
Andy Richter character Eugene Wilson
Lil' Zane character Eric
Denise Dowse character Secretary
Elayne Taylor character Eldon's Wife
Kevin Pollak character Riley / Alligator
Victor Raider-Wexler character Judge B. Duff
Jeffrey Jones character Joe Potter
Mark Griffin character Logger / Nature Show Narrator
Ken Hudson Campbell character Animal Control Officer / Dog / Animal Groupie #3 / Forest Animal (voice)
Anne Stedman character Woman
Tommy Bush character Farmer
Adam Vernier character Farm Worker
Chris Marley character Yuppie Man
Shaun Robinson character Newscaster
Googy Gress character Bear Announcer
Lisa Marie Hugueley character Shamu Trainer
Steve Irwin character The Crocodile Hunter
Doug Seus character Klondike Dave Delivery Man
Louise Osbourne character Waitress
Brie Hill Arbaugh character Chihuahua Lady
E.J. Wessel character Guy Throwing Frisbee
Jacob Vargas character Pepito (voice)
Michael Rapaport character Joey the Raccoon (Voice)
Isaac Hayes character Possum (voice)
Andy Dick character Lennie the Weasel (voice)
Joey Lauren Adams character Squirrel (voice)
Maria Arcé character Fish (voice)
Melique Berger character Fish (voice)
David Cross character Dog / Animal Groupie #2 (voice)
David DeLuise character School Fish #2 (voice)
Trevor Denman character Horse Race Announcer (voice)
John DiMaggio character Seeing Eye Dog / Wassup Fish / Mouse (voice)
Georgia Engel character Giraffe (voice)
Mike Epps character Sonny (voice)
Jamie Kennedy character Bandit / Forest Animal / Animal Groupie #1 (voice)
Tom Kenny character Male Tortoise (voice)
Clyde Kusatsu character Bee (voice)
Cedric the Entertainer character Zoo Bear #1 (voice)
David L. Lander character Bird (voice)
Michael McKean character Bird (voice)
Tara Mercurio character Deer (voice)
Mandy Moore character Girl Bear Cub
Frankie Muniz character Boy Bear Cub (voice)
Bob Odenkirk character Animal Groupie #4 / Forest Animal / Dog (voice)
Phil Proctor character Drunk Monkey (voice)
Reni Santoni character Rat #2 (voice)
Richard C. Sarafian character God Beaver (voice)
Hal Sparks character School Fish #1 (voice)
Renée Taylor character Female Tortoise (voice)
John Witherspoon character Zoo Bear #2 (voice)
Keone Young character Bee (voice)
Jodi Essex character Frisbee Thrower in Park (uncredited)
Lawrence Pressman character Governor of California (uncredited)
